Investigation of Anticholinesterase and Anticancer Effects of Verbascum insulare Boiss. Et Heldr. Extracts Growing in Mus Region
Öz
İnsanlığın varoluşundan bu yana, bitkiler birçok tedavi yönteminde kullanılmıştır. Scrophulariaceae familyasının
bir üyesi olan, Sığırkuyruğu olarakta bilinen Verbascum cinsi üyeleri, çeşitli rahatsızlıklara karşı geleneksel tedavi kullanımlarında değerlendirilmiştir. Bu çalışma ise, Muş yöresinden toplanan bir Verbascum üyesi olan Verbascum insulare Boiss. Et Heldr. 'in biyolojik özelliklerini araştırmaya yöneliktir. V. insulare nin yaprak etanol (L-EtOH), yaprak saf su (L-PW), kök etanol (R-EtOH) ve kök saf su (R-PW) ekstraktları elde edildi. Ekstraktların 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) testi ile HT-29, MCF-7 ve L-929 hücre hatlarına karşı antikanser aktivitesi ve antikolinesteraz aktivitesi incelendi. Etanol ekstraktının kanser hücre hatlarına karşı saf su ekstraktından daha yüksek antikanser aktivite gösterdiği ve daha etkili antikolinerjik etkilere sahip olduğu bulundu. Bu bitkinin antikanser ve antikolinesteraz aktivitesi ile ilgili literatürde daha önce yapılmış bir çalışmaya henüz rastlanılmamıştır. Bu sebeple, çalışmadan elde edilen sonuçlar gelecekte bitkisel ilaç geliştirme çalışmalarına önemli katkılar sunacaktır.
Anahtar Kelimeler
Verbascum insulare Boiss. Et Heldr. Antikolinesteraz Antikanser
Kaynakça
- 1. Pan S.Y., et al. 2013. New perspectives on how to discover drugs from herbal medicines: CAM's outstanding contribution to modern therapeutics. Evidence-Based Complementary and Alternative Medicine, 2013.
- 2. Alan Y. and N Yilmaz. 2019. Phenolic substance contents and biological activities of verbascum insulare boiss. and heldr. Extracts. J steroids, 33: p. 35.
- 3. Süntar I. et al. 2010. An ethnopharmacological study on Verbascum species: from conventional wound healing use to scientific verification. Journal of ethnopharmacology, 132(2): p. 408-413.
- 4. El Gizawy., H.A.E.H., M.A. Hussein. and E Abdel-Sattar. 2019. Biological activities, isolated compounds and HPLC profile of Verbascum nubicum. Pharmaceutical biology, 57(1): p. 485-497.
- 5. Angeloni S., et al.2021. An insight into Verbascum bombyciferum extracts: Different extraction methodologies, biological abilities and chemical profiles. Industrial Crops and Products, 161: p. 113201.
- 6. Talib WH., and A.M Mahasneh. 2010. Antiproliferative activity of plant extracts used against cancer in traditional medicine. Scientia pharmaceutica, 78(1): p. 33-46.
- 7. Zhao Y.-L., et al. 2011. Isolation of chemical constituents from the aerial parts of Verbascum thapsus and their antiangiogenic and antiproliferative activities. Archives of pharmacal research, 34(5): p. 703-707.
- 8. Ellman G.L., et al. 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical pharmacology, 7(2): p. 88-95.
- 9. Almaz Z., et al. 2021. Biological evaluation and molecular docking studies of 4-aminobenzohydrazide derivatives as cholinesterase inhibitors. Journal of Molecular Structure,2021: p. 130918.
- 10. Alkış M.E., et al. 2021. Cobalt and ruthenium complexes with pyrimidine based schiff base: Synthesis, characterization, anticancer activities and electrochemotherapy efficiency. Journal of Molecular Structure, 1226: p. 129402.
- 11. Martins N. and I.C Ferreira. 2017. Neurocognitive improvement through plant food bioactives: A particular approach to Alzheimer’s disease, in Food Bioactives. Springer. p. 267-298.
- 12. Kanbolat S., et al. 2018. Antioxidant, antimicrobial, cytotoxic, anticholinesterase, antityrosinase activities and characterisation of volatile compounds of Verbascum oocarpum by SPME and GC-FID/MS. Journal of Pharmaceutical Research International, 2018: p. 1-12.
- 13. Kahraman C., et al. 2010. Cholinesterase inhibitory and antioxidant properties of Verbascum mucronatum Lam. and its secondary metabolites. Zeitschrift für Naturforschung C, 65(11-12): p. 667-674.
- 14. Iqbal J., et al. 2017. Plant-derived anticancer agents: A green anticancer approach. Asian Pacific Journal of Tropical Biomedicine, 7(12): p. 1129-1150.
- 15. Cragg G.M. and D.J Newman. 2005. Plants as a source of anti-cancer agents. Journal of ethnopharmacology, 100(1-2): p. 72-79.
- 16. Tatlı İ.İ. and Z.S Akdemir. 2006 Cytotoxic activity on some Verbascum species growing in Turkey. Hacettepe University Journal of the Faculty of Pharmacy, (2): p. 77-86.
- 17. Iliescu I.A., et al. 2020. Verbascum nigrum: cytotoxicity evaluation in A431 epidermoid carcinoma cells and untargeted LC‐HR‐MS/MS metabolite profiling. Chemistry & Biodiversity, 17(12): p. e2000644.
- 18. Tauchen J., et al. 2015. In vitro antioxidant and anti-proliferative activity of Ethiopian medicinal plant extracts. Industrial Crops and Products, 74: p. 671-679.
- 19. M Amin H.I., et al. 2020. Phytochemistry of Verbascum Species Growing in Iraqi Kurdistan and Bioactive Iridoids from the Flowers of Verbascum calvum. Plants, 9(9): p. 1066.
Investigation of Anticholinesterase and Anticancer Effects of Verbascum insulare Boiss. Et Heldr. Extracts Growing in Mus Region
Öz
Since the existence of humanity, plants have been used in many treatment methods. Members of the genus Verbascum, also known as mullein, a member of the Scrophulariaceae family, have been evaluated for their traditional use against various ailments. In this study, the anticholinesterase and anticancer activities of Verbascum member Verbascum insulare Boiss. Et Heldr. collected from Muş region were investigated. V. insulare leaf ethanol (L-EtOH), leaf purified water (L-PW), root ethanol (R-EtOH), and root purified water (R-PW) extracts were obtained. The anticancer activity of the extracts against HT-29, MCF-7, and L-929 cell lines was examined using the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test and the anticholinesterase activity was determined. It was found that the ethanol extract showed higher anticancer activity against cancer cell lines than the pure water extract and had more effective anticholinergic influences. No study has been found in the literature on the anticancer and anticholinesterase activity of this plant. Therefore, the results obtained from this study will contribute significantly to the development of herbal medicine in the future.
Anahtar Kelimeler
Verbascum insulare Boiss. Et Heldr. Anticholinesterase Anticancer
Kaynakça
- 1. Pan S.Y., et al. 2013. New perspectives on how to discover drugs from herbal medicines: CAM's outstanding contribution to modern therapeutics. Evidence-Based Complementary and Alternative Medicine, 2013.
- 2. Alan Y. and N Yilmaz. 2019. Phenolic substance contents and biological activities of verbascum insulare boiss. and heldr. Extracts. J steroids, 33: p. 35.
- 3. Süntar I. et al. 2010. An ethnopharmacological study on Verbascum species: from conventional wound healing use to scientific verification. Journal of ethnopharmacology, 132(2): p. 408-413.
- 4. El Gizawy., H.A.E.H., M.A. Hussein. and E Abdel-Sattar. 2019. Biological activities, isolated compounds and HPLC profile of Verbascum nubicum. Pharmaceutical biology, 57(1): p. 485-497.
- 5. Angeloni S., et al.2021. An insight into Verbascum bombyciferum extracts: Different extraction methodologies, biological abilities and chemical profiles. Industrial Crops and Products, 161: p. 113201.
- 6. Talib WH., and A.M Mahasneh. 2010. Antiproliferative activity of plant extracts used against cancer in traditional medicine. Scientia pharmaceutica, 78(1): p. 33-46.
- 7. Zhao Y.-L., et al. 2011. Isolation of chemical constituents from the aerial parts of Verbascum thapsus and their antiangiogenic and antiproliferative activities. Archives of pharmacal research, 34(5): p. 703-707.
- 8. Ellman G.L., et al. 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical pharmacology, 7(2): p. 88-95.
- 9. Almaz Z., et al. 2021. Biological evaluation and molecular docking studies of 4-aminobenzohydrazide derivatives as cholinesterase inhibitors. Journal of Molecular Structure,2021: p. 130918.
- 10. Alkış M.E., et al. 2021. Cobalt and ruthenium complexes with pyrimidine based schiff base: Synthesis, characterization, anticancer activities and electrochemotherapy efficiency. Journal of Molecular Structure, 1226: p. 129402.
- 11. Martins N. and I.C Ferreira. 2017. Neurocognitive improvement through plant food bioactives: A particular approach to Alzheimer’s disease, in Food Bioactives. Springer. p. 267-298.
- 12. Kanbolat S., et al. 2018. Antioxidant, antimicrobial, cytotoxic, anticholinesterase, antityrosinase activities and characterisation of volatile compounds of Verbascum oocarpum by SPME and GC-FID/MS. Journal of Pharmaceutical Research International, 2018: p. 1-12.
- 13. Kahraman C., et al. 2010. Cholinesterase inhibitory and antioxidant properties of Verbascum mucronatum Lam. and its secondary metabolites. Zeitschrift für Naturforschung C, 65(11-12): p. 667-674.
- 14. Iqbal J., et al. 2017. Plant-derived anticancer agents: A green anticancer approach. Asian Pacific Journal of Tropical Biomedicine, 7(12): p. 1129-1150.
- 15. Cragg G.M. and D.J Newman. 2005. Plants as a source of anti-cancer agents. Journal of ethnopharmacology, 100(1-2): p. 72-79.
- 16. Tatlı İ.İ. and Z.S Akdemir. 2006 Cytotoxic activity on some Verbascum species growing in Turkey. Hacettepe University Journal of the Faculty of Pharmacy, (2): p. 77-86.
- 17. Iliescu I.A., et al. 2020. Verbascum nigrum: cytotoxicity evaluation in A431 epidermoid carcinoma cells and untargeted LC‐HR‐MS/MS metabolite profiling. Chemistry & Biodiversity, 17(12): p. e2000644.
- 18. Tauchen J., et al. 2015. In vitro antioxidant and anti-proliferative activity of Ethiopian medicinal plant extracts. Industrial Crops and Products, 74: p. 671-679.
- 19. M Amin H.I., et al. 2020. Phytochemistry of Verbascum Species Growing in Iraqi Kurdistan and Bioactive Iridoids from the Flowers of Verbascum calvum. Plants, 9(9): p. 1066.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Eylül 2022 |
| Gönderilme Tarihi | 27 Aralık 2021 |
| Kabul Tarihi | 29 Nisan 2022 |
| Yayımlandığı Sayı | Yıl 2022 Cilt: 11 Sayı: 3 |
